Pablo BURRACO, Rosa ARRIBAS, Saurabh S KULKARNI, Daniel R BUCHHOLZ, Ivan GOMEZ-MESTRE. Current Zoology. 2015. Glucocorticoids play a key role in mediating stress responses in vertebrates. Corticosterone (CORT) is the main glucocorticoid produced in amphibians, birds, and reptiles, and regulates several metabolic functions. The most common methods for quantifying CORT are competitive binding immunoassays: radioimmunoassay (RIA) and enzyme immunoassay (EIA). RIA has been broadly used since the 1980’s but it requires radioactivity. Leer más.